Draw a map of this gene locus showing all of the gene segments (including leader peptide exon, V exon, D exon, J exon, C exons)

Draw a map of this gene locus showing all of the gene segments (including leader peptide exon, V exon, D exon, J exon, C exons). < pstyle="margin-bottom: 0.0001pt;">
Go to ensemble, find the mouse TCR beta gene. A good starting point is the following:

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http://www.ensembl.org/Mus_musculus/Location/View?db=core;g=ENSMUSG00000076490;r=6:40874232-41600559;t=ENSMUST00000103291

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Chromosome 6: 40,874,232-41,600,559
 
forward strand.

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Question 1
. Draw a map of this gene locus showing all of the gene segments (including leader peptide exon, V exon, D exon, J exon, C exons)

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On the map at the bottom of the page, you can click on individual gene segments, a popup will appear, click on the gene link. For example for TCRB Vb2-201 this is 

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Gene      ENSMUSG00000076462. Now on the left column entitled gene based displays , click sequence this will show you the genomic DNA sequence. introns are black letters and exons are red letters with pink shading. 

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Question 2
. Copy and paste these sequences into microsoft word and assemble the full cDNA of your specific TCRb gene, l ook at the legend below.  Use the genetic code table (available on wikipedia) to find out what the amino acid sequence of this cDNA will be. Assume no P or N nucleotide addition. If your sequence has premature stop codons, you will need to add N nucleotides to the vdj junctions, annotate these. Annotate the amino acids encoded by the junctions of V-D, D-J, J-C. Make sure that your protein has the TCRb transmembrane region which is SATILYEILLGKATLYAVLVSTLVVMAM.

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Draw a map of this gene locus showing all of the gene segments (including leader peptide exon, V exon, D exon, J exon, C exons)

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